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  • 產(chǎn)品名稱： Anti Active Ras Mouse Monoclonal Antibody
  • 產(chǎn)品貨號：
  • 貨期： 0
  • 價格與訂購： 4800
  • 數(shù)量：
    庫存： 5
  • 規(guī)格： 100μg
  • 產(chǎn)品信息
  • 如何訂購
    Catalog Number
    CST26909
    Gene Symbol
    RAS
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    Description
    Anti Active Ras Mouse Monoclonal Antibody
    Background
     Small GTPases are a super-family of cellular signaling regulators. Ras belongs to the Ras sub-family of GTPases that regulate cell proliferation, cell motility, and gene transcription. GTP binding increases the activity of Ras, and the hydrolysis of GTP to GDP renders it inactive. GTP hydrolysis is aided by GTPase activating proteins (GAPs), while exchange of GDP for GTP is facilitated by guanine nucleotide exchange factors (GEFs).
    Immunogen
    Recombinant full length protein of active H-Ras
    Applications
    IP, IHC
    Recommended Dilutions
    1 μg for 1~2 mg total cellular proteins for IP; 
    1:100 dilution for IHC
    Concentration
    1 mg/ml
    Host
    mouse
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    Format
    Liquid
    Clonality
    Monoclonal
    Isotype
    IgG
    Purity
    Purified from ascites
    Preservative
    no
    Constituents
    PBS (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 50% glycerol
    Species Reactivity
    nti active Ras antibody recognizes active H-Ras, N-Ras, and K-Ras from vertebrates.
    Immunoprecipitation/Western Blot

                           IP/WB analysis of active Ras protein. Purified recombinant Ras proteins were loaded with GDP (lane 1) or GTPγS (lane 2). These proteins were immunoprecipitated with anti active Ras mouse monoclonal antibody (Cat. # 26909). After SDS/PAGE, the membrane filter was probed with anti Ras mouse monoclonal antibody (Cat. # 26014).                             
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    Storage Conditions
     Store at -20°C. Avoid freeze / thaw cycles
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    Note
    For Research Use Only.